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2008•Journal of Jinggangshan UniversityRequires access

Determination of roxithromycin by reversed phase high performance liquid chromatography

Deng Jian-an

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Abstract

Objective:To establish a determination method of roxithromycin with reversed phase high perfor-mance liquid chromatography(RT-HPLC).Methods:The RT-HPLC was performed on the Agilent ZORBAX Eclipse XDB-C18 chromatographic column,the mobile phase consisted of acetonitrile-(0.01mol / L) ammoni-um acetate buffer solution(70 : 30,pH = 6.5),the detection wavelength was 220 nm,the column temperature was 40℃.Results: There was a good linear relationship in range of 1.150-100.8 mg / L,r = 0.99977,the average recovery rate was 99.57%;the relative standard deviation was 1.23%(n=5).Conclusion: RP-HPLC determination method of roxithromycin has the advantages of simplicity of operation and speed rapidness.

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Objective:To establish a determination method of roxithromycin with reversed phase high perfor-mance liquid chromatography(RT-HPLC).Methods:The RT-HPLC was performed on the Agilent ZORBAX Eclipse XDB-C18 chromatographic column,the mobile phase consisted of acetonitrile-(0.01mol / L) ammoni-um acetate buffer solution(70 : 30,pH = 6.5),the detection wavelength was 220 nm,the column temperature was 40℃.Results: There was a good linear relationship in range of 1.150-100.8 mg / L,r = 0.99977,the average recovery rate was 99.57%;the relative standard deviation was 1.23%(n=5).Conclusion: RP-HPLC determination method of roxithromycin has the advantages of simplicity of operation and speed rapidness.

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Available abstract

Objective:To establish a determination method of roxithromycin with reversed phase high perfor-mance liquid chromatography(RT-HPLC).Methods:The RT-HPLC was performed on the Agilent ZORBAX Eclipse XDB-C18 chromatographic column,the mobile phase consisted of acetonitrile-(0.01mol / L) ammoni-um acetate buffer solution(70 : 30,pH = 6.5),the detection wavelength was 220 nm,the column temperature was 40℃.Results: There was a good linear relationship in range of 1.150-100.8 mg / L,r = 0.99977,the average recovery rate was 99.57%;the relative standard deviation was 1.23%(n=5).Conclusion: RP-HPLC determination method of roxithromycin has the advantages of simplicity of operation and speed rapidness.

Key concepts: Roxithromycin, Chromatography, High-performance liquid chromatography, Acetonitrile, Chemistry, Relative standard deviation, Phase (matter), Detection limit

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