Solid - Phase Extraction Reverse High Performance Liquid Chromatography in Determining RP - HPLC Concentration in Human Plasma

[Objective]To establish reverse high performance liquid chromatography for determining RP- HPLC concentration in human plasma. [Methods]OASIS solid - phase extraction column was used to extract RP- HPLC in the plasma after basification; reverse high performance liquid chromatography was applied for determination. The chromatographic column was Shim - Pack C 18 (150mm ×4.6mm, 5μm); the protection column was Shim- Pack C18; the mobile phase was 0.1% triethylamine solution (adjusted PH 3.0 with acetic acid) - acetonitrile (55:45, v/v); flow rate was 1.0 ml/min; detective wavelength was 235nm. [Results]The linear relationship was fine within the range of 5 - 100 μg/L ( r =0.9998); the extraction recovery rate was between 91.7% and 105.3%. [Conclusion] This method is rapid, sensitive and accurate which is applicable for clinical drug concentration determination and pharmic study.