Studies on the Anti-influenza Virus Activity of Jinchan Oral Solution in vitro and in vivo

This research intended to study the antiviral activity of Jinchan oral solution against influenza virus in vitro and in vivo.Toxicity concentration of Jinchan oral solution to MDCK cells was assessed by observation of cytopathic effect(CPE).Its inhibitory activity against infiuenza A/FM1/1/47/(H1N1) virus in vitro was determined by hemagglutination assay,and the antiviral activity was compared by using different methods.Viral pneumonia model in ICR mice was used in vivo.The death rate and death protection rate were detected to evaluate the anti-influenza viral effect of Jinchan oral solution.The results showed that Jinchan oral solution had no toxicity to MDCK cells in 1∶128 dilution.The 50% inhibitory dilution of 1∶686 and 1∶740 was calculated when the cells were treated in direct deactivation deliveryⅠ and direct deactivation delivery II respectirely,while no inhibitory activity was found when the cells were treated in precaution delivery.Jinchan oral solution displayed the protective effect against influenza virus in infected mice at the dose of 2.0ml/kg.