Effect of Hydrogen Sulfide Inhibiting Monocyte Chemoattractant Protein-1 Secretion in Human Macrophages Stimulated by Oxidized Low Density Lipoprotein

Objective To explore the effect of hydrogen sulfide(H2S) on oxidized-low density lipoprotein(ox-LDL)-stimulated monocyte chemoattractant protein-1(MCP-1) secretion in THP-1-derived macrophage. Methods THP-1-derived macrophages were divided into 4 groups:normal control group,ox-LDL group,ox-LDL+H2S 100 group and ox-LDL+H2S 500 group.In the normal control group,cells were cultured in the basal medium for 48 hours;in the ox-LDL group,cells were treated with ox-LDL(50 mg·L-1) for 48 hours;in the ox-LDL+H2S 100 group,cells were pretreated with NaHS(100 μmol·L-1) for 30 minutes and then added ox-LDL(50 mg·L-1) to culture for 48 hours;in the ox-LDL+H2S 500 group,cells were pretreated with NaHS(500 μmol·L-1) for 30 minutes and then added ox-LDL(50 mg·L-1) to culture for 48 hours.The content of MCP-1 in cell supernatant was examined by enzyme linked immunosorbent assay. Results Compared with the control group[(34.58±6.77) μmol·L-1],MCP-1 content increased greatly in the ox-LDL group[(66.27±7.29) μmol·L-1],the ox-LDL+H2S 100 group[(49.45±3.08) μmol·L-1] and the ox-LDL+H2S 500 group[(46.64±5.47) μmol·L-1](Pa0.05);compared with the ox-LDL group,MCP-1 content significantly decreased in the ox-LDL+H2S 100 group and the ox-LDL+H2S 500 group(Pa0.05).There was no significant difference of MCP-1 content between the ox-LDL+H2S 100 group and the ox-LDL+H2S 500 group(P0.05). Conclusions Ox-LDL promoted the secretion of MCP-1 in macrophages,while the addition of NaHS suppressed the increase of MCP-1 secretion stimulated by ox-LDL.