Sodium hydrosulfide inhibits inflammatory factor secretion by macrophages

Objective To find out the possible effect of hydrogen sulfide(H2S) on inflammatory factor secretion by macrophage.MethodsTHP-1 monocytes were induced to differentiate into macrophages by incubation with PMA for 24 h.Then the macrophages were divided into four groups randomly: control group,oxidized low density lipoprotein(ox-LDL)50 mg/L group,ox-LDL+ sodium hydrosulfide(NaHS) 100 or 500 μmol/L group.Cells in the control group were treated with neither ox-LDL nor NaHS;cells in the ox-LDL group were treated with 50 mg/L ox-LDL;cells in the ox-LDL+NaHS 100 μmol/L group were treated with both 50 mg/L ox-LDL and 100 μmol/L NaHS;and cells in the ox-LDL+ NaHS 500 μmol/L group were treated with both 50 mg/L ox-LDL and 500 μmol/L NaHS.Cell supernatants in all the above groups were collected after induction for 48 h.ELISA was used to examine the content of tumor necrosis factor-α(TNF-α),interleukin-10(IL-10) and macrophage migration inhibitory factor(MIF) in cell supernatant.Results Compared to the control group,the content of pro-inflammatory factors-TNF-α and MIF increased significantly(P0.05)while the content of anti-inflammatory factor IL-10 decreased significantly in the ox-LDL group(P0.05);compared to the ox-LDL group,the content of TNF-α and MIF decreased greatly(P0.05) while the content of IL-10 increased significantly in the ox-LDL+ NaHS 100 or 500 μmol/L group(P0.05).Conclusions NaHS inhibits the secretion of pro-inflammatory factors-TNF-α and MIF,but promotes the secretion of anti-inflammatory factor-IL-10 while the stimulates of ox-LDL.