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ox-LDL increases endothelial lipase in RAW264.7 macrophages

Wu Xiaoqian, Yi Quan, Lishan He

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Abstract

AIM: To investigate the effect of oxidized low-density lipoprotein(ox-LDL) on the expression of endothelial lipase(EL) in murine RAW264.7 macrophages.METHODS: RAW264.7 cells were incubated with ox-LDL at concentrations of 0～100 mg/L for 24 h.On the other hand,the cells were incubated with or without PDTC(NF-κB inhibitor) for 30 min and then with ox-LDL(50 mg/L) for 24 h.The expression of EL and p65 was detected by Western blotting.RESULTS: The level of EL was significantly increased after ox-LDL incubation in RAW264.7 cells(P0.05).NF-κB was activated by ox-LDL at concentration of 50 mg/L for 15～30 min in RAW264.7 cells.The increase in EL induced by ox-LDL was markedly inhibited by a NF-κB inhibitor PDTC(P0.05).CONCLUSION: ox-LDL significantly increases the expression of EL in RAW264.7 macrophages,which is possibly related to NF-κB activation.

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What this paper is about

AIM: To investigate the effect of oxidized low-density lipoprotein(ox-LDL) on the expression of endothelial lipase(EL) in murine RAW264.7 macrophages.METHODS: RAW264.7 cells were incubated with ox-LDL at concentrations of 0～100 mg/L for 24 h.On the other hand,the cells were incubated with or without PDTC(NF-κB inhibitor) for 30 min and then with ox-LDL(50 mg/L) for 24 h.The expression of EL and p65 was detected by Western blotting.RESULTS: The level of EL was significantly increased after ox-LDL incubation in RAW264.7 cells(P0.05).NF-κB was activated by ox-LDL at concentration of 50 mg/L for 15～30 min in RAW264.7 cells.The increase in EL induced by ox-LDL was markedly inhibited by a NF-κB inhibitor PDTC(P0.05).CONCLUSION: ox-LDL significantly increases the expression of EL in RAW264.7 macrophages,which is possibly related to NF-κB activation.

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Available abstract

AIM: To investigate the effect of oxidized low-density lipoprotein(ox-LDL) on the expression of endothelial lipase(EL) in murine RAW264.7 macrophages.METHODS: RAW264.7 cells were incubated with ox-LDL at concentrations of 0～100 mg/L for 24 h.On the other hand,the cells were incubated with or without PDTC(NF-κB inhibitor) for 30 min and then with ox-LDL(50 mg/L) for 24 h.The expression of EL and p65 was detected by Western blotting.RESULTS: The level of EL was significantly increased after ox-LDL incubation in RAW264.7 cells(P0.05).NF-κB was activated by ox-LDL at concentration of 50 mg/L for 15～30 min in RAW264.7 cells.The increase in EL induced by ox-LDL was markedly inhibited by a NF-κB inhibitor PDTC(P0.05).CONCLUSION: ox-LDL significantly increases the expression of EL in RAW264.7 macrophages,which is possibly related to NF-κB activation.

Key concepts: Incubation, Chemistry, Blot, NF-κB, Lipoprotein lipase, Molecular biology, Lipase, Low-density lipoprotein

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