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2010•Pharmaceutical biotechnologyRequires access

HPLC Determination of Ginsenoside Rg1 in Gucining Tablets

Yao Long-feng

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Abstract

To establish a HPLC method for the content determination of Ginsenoside Rg1 in Gucining tablets,the HPLC was performed on a Merck C18(250mm×4.6mm)chromatography column gradient eluted with acetonitrile-water at the flow rate of 1.0mL /min.The detection wavelength was set at 203nm.Results showed that the linear response range was:0.201～0.603μg/mL,r=0.9994.The mean recoveries was 100.3%(n=9),and RSD=1.34%.This method is simple,quick and accurate.Therefore it is suitable for the quality control of content determination of Ginsenoside Rg1 in Gucining tablets.

About this research paper

What this paper is about

To establish a HPLC method for the content determination of Ginsenoside Rg1 in Gucining tablets,the HPLC was performed on a Merck C18(250mm×4.6mm)chromatography column gradient eluted with acetonitrile-water at the flow rate of 1.0mL /min.The detection wavelength was set at 203nm.Results showed that the linear response range was:0.201～0.603μg/mL,r=0.9994.The mean recoveries was 100.3%(n=9),and RSD=1.34%.This method is simple,quick and accurate.Therefore it is suitable for the quality control of content determination of Ginsenoside Rg1 in Gucining tablets.

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Available abstract

To establish a HPLC method for the content determination of Ginsenoside Rg1 in Gucining tablets,the HPLC was performed on a Merck C18(250mm×4.6mm)chromatography column gradient eluted with acetonitrile-water at the flow rate of 1.0mL /min.The detection wavelength was set at 203nm.Results showed that the linear response range was:0.201～0.603μg/mL,r=0.9994.The mean recoveries was 100.3%(n=9),and RSD=1.34%.This method is simple,quick and accurate.Therefore it is suitable for the quality control of content determination of Ginsenoside Rg1 in Gucining tablets.

Key concepts: Ginsenoside Rg1, Chromatography, Gradient elution, High-performance liquid chromatography, Chemistry, Content determination, Linear range, Elution

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