2009•Journal of Jingmen Technical CollegeRequires access

Determination of Ginsenoside Rg_1 and Notoginsecoside R_1 in Guyuling Tablets by HPLC

Mian Zhang

Abstract

Objective:To establish the determination method of Ginsenoside Rg1 and Notoginsecoside R1 in Guyuling Tablets.Methods: HPLC with C-18 column was used,and the mobile phase was acetonitrile-water(18∶82).The flow rate was 1.0 mL·min-1.The detection wavelength was at 203 nm.Results:The standard curve equation of Ginsenoside Rg1 was Y=2.829X+4.167(r=0.999 9,n=5),the linear range was 3.0~22.5 μg.The standard curve equation of Notoginsecoside R1 was Y=7.580X-3.971(r=0.999 6,n=5),the linear range was 1.0~7.5 μg.The recovery were 98.95% and 99.27% respectively.Conclusion:This method is accurate and sensitive,it can be used for the quality control of Guyuling Tablets.

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Key concepts: Chromatography, High-performance liquid chromatography, Ginsenoside Rg1, Ginsenoside, Linear range, Chemistry, Standard curve, Analytical Chemistry (journal)