Immunohistochemical analysis of TRAIL Protein In carcinogenesis

Objective This study aims to examine if change in death ligand TRAIL expression during different stages of cervical carcinogenesis are related to development of cervical carcinogenesis.Methods Immunohistochemical method was used to detect the expressions of TRAIL in 40 samples of cervical carcinoma,and l8 samples of cervical intraepithelial neoplasia(CIN),together with 12 samples of normal cervical tissues.The level of the expression was analyzed combined with clinicopathological features of age and differentiation degree,histology type and lymph node metastasis.Results In normal cervix and CIN samples,TRAIL staining was mainly observed in the basal/parabasal layer,wherease TRAIL expression was more frequently observed in cervical.The rate of positive expression of TRAIL was 91.67%,83.33% and 55% in normal cervical tissues,cervical intraepithelial neoplasia(CIN) and cervical arcinoma,and the tendency of increase was obvious(p 0.05);there was a significant difference between TRAIL expressions and histologic grades,the lower expression rate of TRAIL expression were found in those poorer differentiated tumors(p 0.01);there was no significant difference between TRAIL expression and age of patient,lymph node metastasis as well as clinic type.Conclusion The deregulation of TRAIL in the CIN-cercical cancer sequence seggest a possible functional role of these death ligands during cervical carcinogenesis.The frequent expression of them presents these death ligands as promiding targets for innovative therapy modalities in cervical cancer.