Study on fermentation conditions of cellulase produced by Trichoderma viride

The fermentation conditions of cellulase produced by Trichoderma viride in the shaking bottle were studied.The results showed that the optimum culture medium conditions were as follows:the most suitable carbon source was bran 4% and corn flour 3%,(NH4)2SO4 0.1%,KH2PO4 0.2%,Tween-80 0.1%;the optimum fermentation conditions were as follows:cultivating temperature 28℃,inoculum concentration 10%,fermentation time 72h.The power of cellulase can reach 12.56U/mL。