2012•Jiepouxue yanjiuRequires access
Effects of bFGF on the expression of GRP78 in cerebral tissue during focal cerebral ischemia in rats
Jiang Hui-b
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Abstract
Objective To investigate the expression of GRP78 in hippocampus and cortex after cerebral ischemia and reperfusion in rats,explore the regulative effects and mechanism of basic fibroblast growth factor(bFGF)to GRP78 on brain tissue.Methods The model of middle cerebral arteries occlusion(MCAO) were performed with intraluminal filament blockade the expression of GRP78 and apoptosis cell in hippocampus and cortex was detected with immunohistochemical method,and TUNEL method.Results No apoptotic cells were observed in sham-operation group,the expression of GRP78 in the cortex and hippocampus tissue of rats was at low level in the sham-operation group.In ischemia-reperfusion group apoptotic neurons in ischemic region of cortex and hippocampus enhanced.The expression of GRP78 was increased in the model group.The decrease of apoptotic neurons in ischemic region of cortex and hippocampus were seen in bFGF treatment group.The expression of GRP78 in the cortex and hippocampus tissue of rats was markably increased in the treatment group than in the model group.Conclusion The results indicate that bFGF depress cell apoptosis,participate in the regulation of expression of GRP78 in ischemic neurons.