Effects of bFGF on the expression of NF-κB in cerebral tissue during cerebral ischemia in rats

Objective To investigate the expression of NF-κB in hippocampus and cortex after cerebral ischemia and reperfusion in rats, explore the regulative effects and mechanism of basic fibroblast growth factor (bFGF)to NF-κB on brain tissue. Methods The model of middle cerebral arteries occlusion (MCAO) were performed with intraluminal filament blockade.the expression of NF-κB and apoptosis cell in hippocampus and cortex was detected with immunohistochemical method、TUNEL method. Results No apoptotic cells were observed in sham-operation group, The expression of NF-κB in the cortex and hippocampus tissue of rats was at low level in the sham-operation group. In ischemia-reperfusion group apoptotic neurons in ischemic region of cortex and hippocampus enhanced.The decrease of apoptotic neurons in ischemic region of cortex and hippocampus were seen in bFGF treatment group. The expression of NF-κB was increased in the model group, The expression of NF-κB in the cortex and hippocampus tissue of rats was markably increased in the treatment group than in the model group. Conclusion The results indicate that bFGF depress cell apoptosis, participate in the regulation of expression of NF-κB in ischemic neurons.