Effects of bFGF on expression of c-Myc in cerebral tissue during focal cerebral ischemia in rats

Objective To investigate the expression of c-Myc in hippocampus and cortex after cerebral ischemia and reperfusion in rats and to explore regulative effects and mechanism of basic fibroblast growth factor(bFGF)to c-Myc in brain tissue.Methods The model of middle cerebral artery occlusion(MCAO) was established with intraluminal filament blockade.The expression of c-Myc and cell apoptosis in hippocampus and cortex were detected with immunohistochemical and terminal deoxyneucleotidyl transferase dUTP nick end labeling(TUNEL) method.Results No apoptotic cell was observed in sham-operation group.The expression of c-Myc in the cortex and hippocampus tissue of rats was at low level in the sham-operation group.In ischemia-reperfusion group,the apoptotic neurons in ischemic region of cortex and hippocampus were enhanced.The decrease of apoptotic neurons in ischemic region of cortex and hippocampus was observed in bFGF treatment group.The grayscale value of the expression of c-Myc was increased in the model group(88.16±2.43,86.72±1.23).The grayscale value of the expression of c-Myc in the cortex and hippocampus tissue was markably decreased in the treatment group than that of in the model group(97.61±1.78,95.35±2.34).Conclusion The results indicate that bFGF depress cell apoptosis,participate in the regulation of expression of c-Myc in ischemic neurons.