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Determination of γ-Amino Butyric Acid in Lactobacillus brevis Fermentation Liquid by HPLC

GE Jing-ping, Cai BaiYan, Song Ming-Ming, Ling Hong-zhi, Song Gang, Wenxiang Ping

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Abstract

High performance liquid chromatography with o-phthaldialdelhyde pre-colomn derivatization was used to determine the content of γ-amino butyric acid (GABA) in Lactobacillus brevis. After pre-colomn derivatization of concentrated fermentation supernatant, separation of GABA was carried out on Nova2pakC18 column with the gradient elution of 50 mmol/L acetate sodium (pH 6.8), methanol and THF (solution A 82:17:1; solution B 22:77:1, V/V). The results of accuracy and recovery rate showed that this menthod can be used to detect trace content of GABA.

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What this paper is about

High performance liquid chromatography with o-phthaldialdelhyde pre-colomn derivatization was used to determine the content of γ-amino butyric acid (GABA) in Lactobacillus brevis. After pre-colomn derivatization of concentrated fermentation supernatant, separation of GABA was carried out on Nova2pakC18 column with the gradient elution of 50 mmol/L acetate sodium (pH 6.8), methanol and THF (solution A 82:17:1; solution B 22:77:1, V/V). The results of accuracy and recovery rate showed that this menthod can be used to detect trace content of GABA.

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Available abstract

High performance liquid chromatography with o-phthaldialdelhyde pre-colomn derivatization was used to determine the content of γ-amino butyric acid (GABA) in Lactobacillus brevis. After pre-colomn derivatization of concentrated fermentation supernatant, separation of GABA was carried out on Nova2pakC18 column with the gradient elution of 50 mmol/L acetate sodium (pH 6.8), methanol and THF (solution A 82:17:1; solution B 22:77:1, V/V). The results of accuracy and recovery rate showed that this menthod can be used to detect trace content of GABA.

Key concepts: Chromatography, Lactobacillus brevis, Chemistry, Butyric acid, Fermentation, Derivatization, High-performance liquid chromatography, Sodium acetate

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