Determination of γ-aminobutyric Acid in Lactic Acid Bacteria Fermented Liquid by HPLC

Determine the contents of γ-aminobutyric acid in lactic acid bacteria fermented liquid by HPLC with OPA pre-column derivatization-UV Spectrophotometry.After centrifugation and pre-column derivatization of lactic acid bacteria fermentation liquid,the separation and detection of γ-aminobutyric acid is carried out by Luna-C18 column(250×4.6 mm,5 μm),mobile phase is 15 mmol/mL of sodium acetate buffer solution∶methanol of 55∶45,the flow rate is 0.8 mL/min,the column temperature is 30 ℃,the UV detection wavelength is 334 nm.The results show that the contents of γ-aminobutyric acid appear good linear relationship within 50~800 μg/mL,the linear equation is y=5465.9x-5411(R2=0.9996),and the detection limit is 2.6714 μg/mL,the average rate of recovery is 98.31%,RSD is 2.27%.This method has simple and fast operation,good precision and isolation,and it meets the demand of trace element analysis.