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2006•Research and Practice on Chinese MedicinesRequires access

Determination of Chlorgenic Acid in Yinqiao Mixture by RP-HPLC

Jin Yang

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Abstract

Objective To establish the determination method of chlorogenic acid in Yinqiao mixture by RP-HPLC.Methods The separation was performed on C_(18) column with acetonitrile-0.1%phosphoric acid(11:89) as mobile phase and 1.0 mL·min~(-1)of the flow rate,the detection wavelength was at 327 nm,the column temperature was room temperature.Results The linear range of chlorogenic acid was in the range of 0.1226～1.225 6 μg,r=0.999 9.The average recovery was 99.16%,and RSD was 0.76%(n=5).Conclusion This method was rapid,sensitive and accurate,it can be used for quality control of Yinqiao mixture.

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What this paper is about

Objective To establish the determination method of chlorogenic acid in Yinqiao mixture by RP-HPLC.Methods The separation was performed on C_(18) column with acetonitrile-0.1%phosphoric acid(11:89) as mobile phase and 1.0 mL·min~(-1)of the flow rate,the detection wavelength was at 327 nm,the column temperature was room temperature.Results The linear range of chlorogenic acid was in the range of 0.1226～1.225 6 μg,r=0.999 9.The average recovery was 99.16%,and RSD was 0.76%(n=5).Conclusion This method was rapid,sensitive and accurate,it can be used for quality control of Yinqiao mixture.

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Available abstract

Objective To establish the determination method of chlorogenic acid in Yinqiao mixture by RP-HPLC.Methods The separation was performed on C_(18) column with acetonitrile-0.1%phosphoric acid(11:89) as mobile phase and 1.0 mL·min~(-1)of the flow rate,the detection wavelength was at 327 nm,the column temperature was room temperature.Results The linear range of chlorogenic acid was in the range of 0.1226～1.225 6 μg,r=0.999 9.The average recovery was 99.16%,and RSD was 0.76%(n=5).Conclusion This method was rapid,sensitive and accurate,it can be used for quality control of Yinqiao mixture.

Key concepts: Phosphoric acid, Chromatography, Chlorogenic acid, Chemistry, High-performance liquid chromatography, Linear range, Acetonitrile, Analytical Chemistry (journal)

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