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Optimization of preparing conditions of soy peptide

Xingfeng Guo

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Abstract

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate.On the basis of single factors analysis,central composite design with four variables and Response Surface Methodology(RSM) were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate(SPI).Statistics Analysis System(SAS) was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase were temperature 41.4℃,pH7.0,the ratio of enzyme and substrate 0.84%,substrate concentration 8.43%,and hydrolysis time 3 h.The predict hydrolysis degree was 11.09%,under these conditions the test hydrolysis degree was 11.08%.

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What this paper is about

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate.On the basis of single factors analysis,central composite design with four variables and Response Surface Methodology(RSM) were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate(SPI).Statistics Analysis System(SAS) was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase were temperature 41.4℃,pH7.0,the ratio of enzyme and substrate 0.84%,substrate concentration 8.43%,and hydrolysis time 3 h.The predict hydrolysis degree was 11.09%,under these conditions the test hydrolysis degree was 11.08%.

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Available abstract

Bacillus subtilis As1.398 neutral proteinase was used to hydrolyze soybean protein isolate.On the basis of single factors analysis,central composite design with four variables and Response Surface Methodology(RSM) were applied to optimize the enzyme hydrolysis conditions of soybean protein isolate(SPI).Statistics Analysis System(SAS) was used to process the test results.The optimum hydrolysis conditions for Bacillus subtilis As1.398 neutral proteinase were temperature 41.4℃,pH7.0,the ratio of enzyme and substrate 0.84%,substrate concentration 8.43%,and hydrolysis time 3 h.The predict hydrolysis degree was 11.09%,under these conditions the test hydrolysis degree was 11.08%.

Key concepts: Hydrolysis, Bacillus subtilis, Response surface methodology, Soy protein, Enzymatic hydrolysis, Substrate (aquarium), Central composite design, Chemistry

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