Research on Technology of Extracting Collagen from Fish Scales of Wuchang Fish

[Objective] The study aimed to explore the optimum technology of extracting the collagen from the scales of Wuchang fish.[Method] With the scales of Wuchang fish as the raw materials,adopting different concn.of hydrochloric acid as the decalcification solution,the collagen was extracted by using 0.5 mol/L acetic acid-sodium acetate buffer solution.With the extraction rate of the collagen as the index,4 factors of the decalcification time,acid concn.for the decalcification,solid-liquid ratio and extraction time were selected as the impact factors to conduct the orthogonal test so as to confirm the optimum technology conditions of extracting the collagen from the scales of Wuchang fish.[Result] The orthogonal test shows that when the extraction temperature was at 12 ℃,the impact degree of 4 factors on the extraction rate of the collagen from the scales of Wuchang fish was in order of solid-liquid ratio ﹥ decalcification time ﹥acid concn.for decalcification ﹥ extraction time.The optimum technology conditions for extracting the collagen from the fish scales were as follows: solid-liquid ratio of 1∶ 25,decalcification time of 3 h,extraction time of 1.5 h and acid concn.for decalcification being 0.4 mol/L,under which,the extraction rate of the collagen was 1.142%.[Conclusion] The study provided the reference basis for the development and application of the collagen from the fish scales.