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EXPRESSION OF HUMAN RECOMBINANT HIV-1 gp41 TRANSMEMBRANE PROTEIN IN E.coli
Jing Wang, Bin Wang, Yongbo Lu, Qiubo Wang, Yanli Zhang
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Abstract
Objective To construct the prokaryotic expression plasmid of recombinant HIV 1 gp41 gene fragment of SF2 strain and express in Methods The target gene was amplified by PCR ,and the restricted inserting of the target gene into the prokaryotic expression vector pMAL p2 was finished by restricted endonuclease. The constructed recombinant prokaryotic expression plasmid was transformed into E.coli.DH5α., and the inserted gene was then expressed by inducing with IPTG and the prokaryotic expression products were analyzed by SDS PAGE electrophoresis. Results Recombinant prokaryotic expression plasmid was gained and the prokaryotic expression products of HIV 1 gp41 gene were acquired. Conclusion The constructed HIV 1 gp41 recombinant plasmid in pMAL p2 could be expressed in E.coli.DH5α. [