CONSTRUCTION AND EXPRESSION OF THE PROKARYOTIC EXPRESSION CLONE OF HSV-1 ENVELOPE GLYCOPROTEIN G

Objective To construct the prokaryotic expression plasmid of glycoprotein G Us4 gene of HSV 1 strain Stoker. Methods The target gene was amplified by PCR, and the restricted insertion of the target gene into the prokaryotic expression plasmid PGEX 4T 2 was finished by restricted endonuclease. Results Recombinant prokaryotic expression plasmid was gained. Conclusion Recombinant prokaryotic expression plasmid was confirmed by restricted endonuclease analysis and the recombinant protein was confirmed by SDS PAGE.