Cloning and expression of recombinant human fertilization antigen-1 in E. coli.

Objective:To determine the anti-sperm antibody in serum of infertility patients,a recombinant prokaryotic expression plasmid of human fertilization antigen-1 was constructed and the FA-1 protein was expressed in E.coli. Methods:The code gene of human FA-1 was amplified by reverse transcript PCR from human testes organism and cloned into a prokaryotic expression vector pBV220. The recombinant plasmid was identified by restriction enzyme digestion and DNA sequencing. The recombinant plasmid was transfected into E.coli JM109,then the recombinant protein was expressed in E.coli and detected by using SDS-PAGE. Results:The sequence of recombinant human FA-1 was correct. The relative molecular mass of the expressed product was identical to that of expectation of 15KDa. Conclusion:The code gene of human FA-1 has been successfully cloned and expressed in E.coli.