Analysis on outbreak of food poisoning induced by Multi-serotype Vibrio parahaemolyticus with pulsed-field gel electrophoresis typing

Objective:To study the molecular epidemiology and homology relationship among 7 strains of Vibrio parahaemolyticus isolated from patients samples during food poisoning outbreak with routine experiment and pulsed-field gel electrophoresis(PFGE)typing,to trace the source of infections so as to support the ascertainment of epidemic control strategy.Methods: Strains isolated from samples as mentioned above were determined according to the method of GB/T 4789.7-2008.Biochemical identification,Kanagawa test,serological typing and PFGE were applied to type of the strains.Results: 7 strains with serological typing were belonged to 3 patterns,such as 5 strains of O3∶ K6,1 strain of O4∶ K8 and O1∶ K56,respectively.6 strains with Kanagawa test were positive.And PFGE result showed 3 patterns respectively.Conclusion: It indicates that mixed infection of different clone groups of vibrio parahaemolyticus with O3∶ K6 as the main type may lead to the food poisoning in this case.