PFGE analysis on multiple serotypes Vibrio parahaemolyticus of food poisoning

[Objective]By using the traditional culture method and pulsed-field gel electrophoresis(PFGE) typing technique,to isolate the strains of food poisoning cases caused by multiple serotypes Vibrio parahaemolyticus,compare their serotype,virulence,drug resistance and homology.[Methods]After the conventional identification,serological typing,analysis of virulence and drug resistance in the strains of Vibrio parahaemolyticus,PFGE was conducted to identify their molecular typing.[Results]A total of 8 strains of Vibrio parahaemolyticus were isolated from food poisoning patients,which included 4 strains of O3: K6 and 3 strains of O4: K8,while 1 case failed to typing.PFGE clustering research showed that those strains belonged to five distinct PFGE type.The isolated strains were resistant to ampicillin and positive for thermostable direct hemolysin gene(TDH).[Conclusion]PFGE clustering analysis concluded that this food poisoning event is caused by different Vibrio parahaemolyticus,and all strains are positive for TDH.PFGE typing is more suitable for homologous analysis of strains and infectious source tracking.