Observation on the Relationship between K-ras Mutation in Pancreatic Juice Accompanying by CA19-9 Level and Recurrence of Pancreatic Carcinoma

Objective:To study the relationship between K-ras mutation in pancreatic juice accompanying by CA19-9 level and recurrence of pancreatic carcinoma.Methods:K-ras point mutation was detected using the polymerase chain reaction and restriction fragment-length polymorphism(PCR-RFLP) in pure pancreatic juice(PPJ),which were collected from the pancreatic togarphy(ERCP).And CA19-9 levels were examined in 32 patients with pancreatic carcinoma.The relationships between K-ras and CA19-9 level and recurrence rate were analyzed.Results:(l) The positivity of K-ras point mutation in PPJ was 54.17%,the positivity of K-ras point mutation in PPJ correlated with tumor recurrence rate(P0.05).(2) The recurrence rate in high CA19-9 level group with positive K-ras point mutation in PPJ was 62.50%,while in hypo CA19-9 level was 30.0%,(P0.05).Conclusion:K-ras mutation in pancreatic juice and CA19-9 level are effective in dictators for recurrence and prognosis of pancreatic carcinoma.The analysis of multi-gene is more valuable than that of single.