Effect of ligustrazine on the signal transduction of the recovery of bone marrow microenvironment in irradiation-injured mice

Objective To evaluate the effect of Ligustrazine on bone marrow hematopoietic microenvi\|ronment and the mechanism of signal transduction in radiation\|injured mice. Methods After irradiation by 6 0 Gy 60 Co γ\|ray,each mouse was given orally by 4 mg Ligustrazine twice a day for 13 days.On the 3th,7th and 14th day,histopathologic sections from each paraffin\|embeded ulnae were stained with anti\|factor Ⅷ\|related antigen and anti\|flk\|1.The femura were taken and the bone marrow nuclear cell suspension was made to culture bone marrow stromal cells(BMSCs),and the expression of phosphorylated FAK on BMSCs was assayed by Western blot. Results The recovery of hematopoiesis in the Ligustrazine\|treated group was sooner than the control group.The expression levels of flk\|1 and phosphorylated FAK in the Ligustrazine\|treated group were significant higher than the control group( P 0 05). Conclusion The Ligustrazine can enhance the expression of flk\|1 on bone marrow endothelial cells and the expression of phosphorylated FAK on BMSCs.This may be one of mechanisms of Ligustrazine to promote recovery of hematopoiesis after γ\|irradiation.\;