Effect of Ligustrazine on IL - 6 and IL - 18 Gene Expression in Bone Marrow Cell of Blood Deficiency Mice Induced By Radiation

Objective:To study the effect of ligustrazine on IL-6 and IL-18 gene expression in bone marrow cell of blood deficiency mice induced by radiation and provide the basis for clarifying the molecular mechanism of blood enriching and immunoregulatory function of ligustrazine.Methods:The blood deficiency mice were induced by using a single dose of 3.5 Gy60Co γ radiation.Peripheral blood leukocytes were counted and the thymus and spleen indexes were calculated.IL-6 and IL-18 mRNA in bone marrow were measured by RT-PCR.Results:Ligustrazine can significantly raise the peripheral blood leukocytes number(P 0.05) and the spleen index(P 0.01) of blood deficiency mice induced by radiation and up-regulate IL-6(P 0.05) and IL-18(P 0.01) gene expressions of bone marrow cell of the mice,but had no significant effect on thymus index of the mice.Conclusion:Ligustrazine can promote IL-6 and IL-18 gene expression in bone marrow cells,which is probably one of the mechanisms of blood proliferation and immunoregulatory function of the ligustrazine.