Application of β-cyclodextrin derivatives as mobile phase additives on cellulose triphenylcarbamate chiral cohmm

Cellulose triphenylcarbamate(CTPC) was synthesized. Elemental analysis and IR spectrum indicated that most of the hy-droxy groups of the microcrystalline cellulose had been converted into ester groups.The silanized gel was coated with CTPC by using tetrahydrofuran as solvent. The enantiomeric resolution of phenylalanine and tryptophan on high performance liquid chro-matography was achieved, when β-cyclodextrin( β-CD), dimethyl β-cyclodextrin(DM-β-CD) and trimethylβ-cyclodextrin(TM-β-CD) were used as mobile phase additives. CTPC column for separation of phenylalanine and tryptophan possessed a new synergis- tic effect--two-phase synergistic effect. From the principle of supramolecular chemistry, we assumed that the effect was based on the supramolecular action of chiral stationary phase and chiral mobile phase additive.