Application of β-cyclodextrin derivative on cellulose tribenzoate chiral columns

Cellulose tribenzoate (CTB) was synthesized. Elemental analysis and IR spectra indicated that most of the hydroxy groups of the microcrystallin cellulose were converted into ester groups. The silanized gel was coated with CTB by using tetrahy drofuran as solvent, which was used as the swtationary phase of high performance liquid chromatography. When β-cyclodextrin(β-CD), dimethyl β-cyclodextrin (DM-β-CD) and trimethyl β-cyclodextrin (TM-β-CD) were used as mobile phase additives, the separation of enantiomers of phenylalanine and tryptophyan showed two-phase synergistic effects.