OBSERVATION OF EFFICACY OF VAPOR HYDROGEN PEROXIDE LOW-TEMPERATURE STERILIZER IN DISINFECTION OF BIOSAFETY LABORATORY

Objective To observe the efficacy of vapor hydrogen peroxide low-temperature sterilizer(VHPLTS) STERIS VHP 1000ED in disinfection of biosafety laboratory.Methods Chemical indicators and biological indicators were adopted to inspect the efficacy of the sterilizer in disinfection of air and surfaces in biosafety laboratory.Results In laboratory,at ordinary temperature and relative humidity between 40% to 50%,VHPLTS was adopted to make the space distribution of hydrogen peroxide concentration about 640 mg/m3.After 90 min,the color of all chemical indicators changed from orchid to ecru which is the qualified color.Under the same condition,successive test for 4 times showed that all Bacillus stearothermophilus biological indicator cultures were negative,all the plates inoculated with Bacillus subtilis var.niger in experiment group slowed no bacteria growth,while the positive control grew normally.All the biological monitoring was eligible.Conclusions The vapor hydrogen peroxide low-temperature sterilizer,STERIS VHP 1000ED,has good efficacy in disinfection of indoor air and surfaces in laboratory with safe manipulation.