OBSERVATION ON STERILIZATION EFFICACY OF HYDROGEN PEROXIDE VAPOR IN BIOSAFETY LABORATORY
Tie Jin
Abstract
Tie Jin
Abstract
Objective To observe the sterilization efficacy of hydrogen peroxide vapor on hard surface and air in biosafety laboratory.Methods Spores of Bacillus subtilis var.niger was used as indicating microorganism,and the sterilization efficacy was observed in different hydrogen peroxide dosage,killing time,and space by carrier quality biocide test.Results BIOQUELL sterilizer continuously sprayed 346.5 g/L hydrogen peroxide vapor by 12 ml/m3 for 60 min,all of the carrier with B.subtilis var.niger spores which were put at different positions in the biosafety laboratory were killed completely.Conclusion Hydrogen peroxide vapor has very good sterilization efficacy on the biosafety laboratory.
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Objective To observe the sterilization efficacy of hydrogen peroxide vapor on hard surface and air in biosafety laboratory.Methods Spores of Bacillus subtilis var.niger was used as indicating microorganism,and the sterilization efficacy was observed in different hydrogen peroxide dosage,killing time,and space by carrier quality biocide test.Results BIOQUELL sterilizer continuously sprayed 346.5 g/L hydrogen peroxide vapor by 12 ml/m3 for 60 min,all of the carrier with B.subtilis var.niger spores which were put at different positions in the biosafety laboratory were killed completely.Conclusion Hydrogen peroxide vapor has very good sterilization efficacy on the biosafety laboratory.
Key concepts: Hydrogen peroxide, Sterilization (economics), Bacillus subtilis, Spore, Biosafety, Chemistry, Microbiology, Biology