Design of the best dosage of vapor hydrogen peroxide in disinfection of biosafety laboratory

Objective To design the best dosage of vapor hydrogen peroxide in disinfection of high pathogenic microorganism biosafety laboratory. Methods One room of 120 m3 in biosafety laboratory was selected. Hydrogen peroxide solution was vaporized by hydrogen peroxide sterilizer( VHP). Nine types of vapor hydrogen peroxide inject speed and duration time were used to disinfect. After the disinfection,the chemical indicators and biological indicators were placed on 10 locations in the room to observe the color of chemical indicators and the growth condition of all the plates inoculated with Bacillus subtilis var niger. After the 9 types of disinfection,the minimum vapor hydrogen peroxide was determined and then repeated for 6 times in order to verify the disinfection effect. Results Qualified disinfection was proved that the color of the chemical indicators changed from orchid to ecru when the dosage no less than 5 g / M3. The minimum vapor hydrogen peroxide was determined at 5 g / m3. After 6 times repeation of disinfection experiment at 5 g / m3,all the disinfection results were qualified. Conclusions The best dosage of hydrogen peroxide solution with concentration of 35% was 5 g / m3. It can reach the good efficacy in disinfection of air and surfaces in biosafety laboratory,which was suit for the disinfection in high pathogenic microorganism biosafety laboratory.