Studies on Residue Analysis Method of Tebuconazole in Soybean by Gas Chromatography

[Objective] The aim was to establish a residue analysis method of tebuconazole in soybean by gas chromatography.[Method] After samples went extracted by acetone,cleaned up with petroleum ether by liquid-liquid partition and Florisil siliceous earth chromatography column,they were determined by gas chromatography with nitrogen-phosphorus detection(NPD).[Result] Average recoveries of tebuconazole in soybean and plant were 90.7%-105.3% and 93.2%-100.6% respectively under the spiking level of 0.02-1.00 mg/kg,and the corresponding relative standard deviations were 2.1%-8.9% and 1.7%-4.6%,respectively.The lowest detection limit of instrument for tebuconazole was 1×10-10 g,and the limit od quantitation was 0.01 mg/kg.[Conclusion] The method is sample sensitive and accurate,furthermore its sensitivity,precision and detection limit meet the requirement of pesticide residue analysis.