Protective effect of liver ischemic preconditioning on the extrahepatic organs injury induced by liver ischemia/repurfusion in rats

Objective To study the protective effect of liver ischemic preconditioning on the extrahepatic organs injury induced by liver ischemia/repurfusion in rats. Methods Seventy-two Sprague-Dawley rats were randomly assigned into group IP,group I/R and group S (sham-operation group), each group had 24 rats. After ischemic preconditioning and ischemia/repurfusion animal models were set up,the pathological changes of small intestine, pancreas, myocardium, kidney, lung, brain and skeletal muscle tissues were observed at 2h,24h and 1week,respealively. Results (1) The degree(s) of small intestinal injury: at 2h and 24h, The injury in group IP and group I/R were significantly higher than that in group S (all P 0.01); and at 2h, it in group I/R was significantly higher than that in group IP ( P 0.05). (2) The degree of renal injury: in group I/R, this was significantly higher than that in group S at 2h, 24h and 1 week (P 0.05, P 0.01 and P 0.05, respectively); and at 24h and 1 week, in group IP it was significantly lower than that in group I/R ( P 0.01 and P 0.05, respectively). (3) Although the injury of the pancreas and lung in group IP were lighter than those in group I/R, no statistical differences showed between the 2 groups ( P 0.05);but in group I/R and IP group were significantly severe than those in group S( P 0.05). And no obvious injuries were observed on the brain, myocardium and skeletal muscle tissues in all the 3 groups. Conclusions Liver ischemic preconditioning can effectively ameliorate the injuries of the small intestine and kidney induced by liver ischemia/reperfusion in rats.