The expression and clinical singlificance of PTEN gene protein in large colorectal benign and malignant tumour

Objective To explore the expression and clinical significance of PTEN gene in large colorectal benign and malignant tumorMethods Immunohistochemical S-P technique was used to determine the expression of PTEN protein in 28 cases of human normal large colorectal mucosa tissue,27 cases of colorectal adenoma and adjacent nontumorous tissue,12 cases of colorectal polyps and adjacent tissue,48 cases of colorectal carcinoma and adjacent nontumorous tissue.Results The expression level of PTEN protein in all large colorectal benign and malignant tumor,especial colorectal polyps and colorectal adenomacarcinoma,were remarkably lower than that in normal large colorectal mucosa tissue and adjacent tissue.There was significantly negative correlation between the expression level of PTEN protein in colorectal polyps and colorectal carcinoma and the volume of the tumour.The expression of PTEN in carcinoma was significantly correlated to its histology differentiation degree,but not to its clinical Dukes stage,lymph node and distant organs metastasis.Conclusion The results suggest that tumor suppressor gene PTEN may play an important inhibitory role in the oncogenes is and development of colorectal polyps and colorectal carcinoma,and imply that the detection of the expression of PTEN protein may be of great value for the early diagnosis of colorectal polyps and colorectal carcinoma,and for guiding the further clinical treatment after operation and the judgment of prognosis.