The expression and the significance of PTEN and Caspase-3 in colorectal carcinoma.

Objective To determine the expression of PTEN and Caspase-3 in colorectal carcinoma and investigate the relationship between them and the clinicopathologic features.Methods The S-P immunohistochemical method was used to detect the expression and significance of PTEN and Caspase-3 in colorectal tissues.Results Expressions of PTEN in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers are 100%、70% and 51.61%,respectively.Expression of PTEN in paracarcinoma mucosa is 60.87%,similar to that in colorectal cancer.Expression of PTEN in colorectal cancer is lower than that in non-cancer groups(P 0.05).Expressions of Caspase-3 in normal colorectal mucosa tissues,colorectal adenoma tissues and colorectal cancers are 93.33%、60%、45.16%,respectively.And expression in paracarcinoma mucosa is 52.17%.Expression of Caspase-3 in normal colorectal mucosa tissue is higher than that in colorectal adenoma tissue and colorectal cance(rP 0.05).The expressions of PTEN and Caspase-3 in well-differentiated adenocarcinoma were higher than that in poorly-differentiated adenocarcinoma(P 0.05).Also,the lost or decreased expression of PTEN significantly correlated with clinicalpathological stage .Significant relationships were found between PTEN and Caspase-3(r =0.675,P 0.01).Conclusion The experiment suggests that PTEN and Caspase-3 may play certain roles in the oncogenesis and progression of colorectal cancer.Their expressions may be favorable markers and useful prognostic indicators in the patients with colorectal cancer.