Determination of Astragaloside IV and paeoniflorin in total glucosides of Buyang Huanwu decoction by HPLC

Objective To establish the HPLC conditions for determination of Astragaloside Ⅳ and paeoniflorin in total glucosides of Buyang Huanwu decoction.Methods The Kromasil C18 column(250 mm×4.6 mm,5μm) was used.Astragaloside Ⅳ detection conditions:Evaporative light scattering detector was used;the mobile phase was methanol-water(80:20)(v/v);temperature of drift tube was 60 ℃;gas flow rate was 1.5 L/min;pressure was 0.5 Mpa and column temperature was 30 ℃.Paeoniflorin detection conditions:the mobile phase was methanol-water(23:77)(v/v);the flow rate was 1.0 mL/min;the detection wavelength was 218 nm and column temperature was 30 ℃.Results Astragaloside IV was better in linear correlation between 0.57~9.10 μg,Y=1.5063X +2.4096,(r=0.9994).Paeoniflorin was better in linear correlation between 0.48~7.70 μg,Y=598.91X-2.3346(r=0.9999).The average recoveries were 99.62% and 101.3% respectively,and RSD were 2.6% and 1.7%(n=6) respectively.Conclusion The method is rapid,simple and accurate and has high sensitivity for determination of Astragaloside Ⅳ and paeoniflorin,which can provide quality standards for the study of total glycosides.