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2001•The Journal of Cervicodynia and LumbodyniaRequires access

Analysis of GDNFmRNA gene expression after crushed spinal cord injury in rats

Song Hai

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Abstract

Objective To investigate the gene expressing of GDNFmRNA after crushed spinal cord injury(SCI)in rats.Methods The specimen of spinal cord were taken at different time after T 13 segments of spinal cord were injured by modified Allen crush method(10g×7.5cm).The level of GDNFmRNA was observed and compared before and after SCI,using semi quantitative RT PCR method with β Actin as a inner consult.Results Much minim expression of GDNFmRNA was found in spinal cord in normal adult rats,but it increased 5 times at 24 hours after SCI,20 times at 72 hours,4 times at 7 days and still higher than normal level 10 days after SCI.Conclusion It should be one kind of self protective behave models for spinal cord to express high standard GDNFmRNA in the early stage after SCI.It might require large amount of GDNF for neuron to be repaired GDNF should be supplied early when it was applied to treat SCI.

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Objective To investigate the gene expressing of GDNFmRNA after crushed spinal cord injury(SCI)in rats.Methods The specimen of spinal cord were taken at different time after T 13 segments of spinal cord were injured by modified Allen crush method(10g×7.5cm).The level of GDNFmRNA was observed and compared before and after SCI,using semi quantitative RT PCR method with β Actin as a inner consult.Results Much minim expression of GDNFmRNA was found in spinal cord in normal adult rats,but it increased 5 times at 24 hours after SCI,20 times at 72 hours,4 times at 7 days and still higher than normal level 10 days after SCI.Conclusion It should be one kind of self protective behave models for spinal cord to express high standard GDNFmRNA in the early stage after SCI.It might require large amount of GDNF for neuron to be repaired GDNF should be supplied early when it was applied to treat SCI.

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Available abstract

Objective To investigate the gene expressing of GDNFmRNA after crushed spinal cord injury(SCI)in rats.Methods The specimen of spinal cord were taken at different time after T 13 segments of spinal cord were injured by modified Allen crush method(10g×7.5cm).The level of GDNFmRNA was observed and compared before and after SCI,using semi quantitative RT PCR method with β Actin as a inner consult.Results Much minim expression of GDNFmRNA was found in spinal cord in normal adult rats,but it increased 5 times at 24 hours after SCI,20 times at 72 hours,4 times at 7 days and still higher than normal level 10 days after SCI.Conclusion It should be one kind of self protective behave models for spinal cord to express high standard GDNFmRNA in the early stage after SCI.It might require large amount of GDNF for neuron to be repaired GDNF should be supplied early when it was applied to treat SCI.

Key concepts: Medicine, Glial cell line-derived neurotrophic factor, Spinal cord injury, Spinal cord, Cord, Anesthesia, Anatomy, Surgery

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