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2003•Qianwei Journal of MedicineRequires access

Analysis of GDNFmRNA expression after firearm wound of spinal cord in rats

Z Chen

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Abstract

Objective To investigate the gene expression of Glial cell line-derived heurotrophic factor (GDNF) mRNA on T12～L1 spinal cord after firearm wound of spinal cord(SCI) in rats. Methods The specimen of T12～L1 spinal cord was taken respectively before and after SCI. The level of GDNF mRNA on spinal cord was observed and compared before and after SCI,using semi-quantitative RT-PCR method with β-actin as a inner consult. Results Minim expression of GDNF mRNA was found on spinal cord before SCI, but it decreased 25%,71%,82%,86% on 1st d, 4th d, 7th d and 10th d respectively. Conclusion The reduction of GDNF mRNA level on homologous segmental spinal cord would be caused by losing the support of GDNF mRNA from the target tissue through sciatic nerve, thus providing one of theory foundations for foreign GDNF to be used in treating SCI.

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Objective To investigate the gene expression of Glial cell line-derived heurotrophic factor (GDNF) mRNA on T12～L1 spinal cord after firearm wound of spinal cord(SCI) in rats. Methods The specimen of T12～L1 spinal cord was taken respectively before and after SCI. The level of GDNF mRNA on spinal cord was observed and compared before and after SCI,using semi-quantitative RT-PCR method with β-actin as a inner consult. Results Minim expression of GDNF mRNA was found on spinal cord before SCI, but it decreased 25%,71%,82%,86% on 1st d, 4th d, 7th d and 10th d respectively. Conclusion The reduction of GDNF mRNA level on homologous segmental spinal cord would be caused by losing the support of GDNF mRNA from the target tissue through sciatic nerve, thus providing one of theory foundations for foreign GDNF to be used in treating SCI.

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Available abstract

Objective To investigate the gene expression of Glial cell line-derived heurotrophic factor (GDNF) mRNA on T12～L1 spinal cord after firearm wound of spinal cord(SCI) in rats. Methods The specimen of T12～L1 spinal cord was taken respectively before and after SCI. The level of GDNF mRNA on spinal cord was observed and compared before and after SCI,using semi-quantitative RT-PCR method with β-actin as a inner consult. Results Minim expression of GDNF mRNA was found on spinal cord before SCI, but it decreased 25%,71%,82%,86% on 1st d, 4th d, 7th d and 10th d respectively. Conclusion The reduction of GDNF mRNA level on homologous segmental spinal cord would be caused by losing the support of GDNF mRNA from the target tissue through sciatic nerve, thus providing one of theory foundations for foreign GDNF to be used in treating SCI.

Key concepts: Glial cell line-derived neurotrophic factor, Spinal cord, Medicine, Spinal cord injury, Messenger RNA, Sciatic nerve, Anatomy, Anesthesia

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