Analysis of GDNFmRNA Gene Expression on Sciatic Nerve after Crushed Spinal Cord Injury in Rats

Objective: To investigate the gene expression of glia cell line derived neurotrophic factor GDNFmRNA on sciatic nerve after crushed spinal cord injury(SCI) in rats.Methods:The speciment of sciatic nerve was taken at different time before and after T 13 segments of spinal cord were injured by modified Allen crush method (10g×7.5cm).The level of GDNFmRNA was observed and compared before and after SCI,using semi quantitative RT PCR method with β Actin as an inner consult.Results:Much minim expression of GDNFmRNA was found in sciatic nerve of normal adult rats,but it increased 4 times 24 hours after SCI,15 times 72 hours,3 times 7 days and slight higher than normal level 10 days after SCI.Conclusion:It should be one kind of self protective behave models for sciatic nerve to express high standard GDNFmRNA through neuron axon target route in the early stage after SCI.GDNF should be supplied early when it was applied to treat SCI.