2012•Chemistry & BioengineeringRequires access

Resonance Light Scattering Method for the Determination of Deoxyribonucleic Acid with Orange G

Hao Gui-qing

Abstract

The resonance light scattering(RLS) spectra of orange G(OG) with deoxyribonucleic acid have been studied.The RLS of OG is greatly enhanced by deoxyribonucleic acid,and at a specific wavelength(356 nm),the enhanced intensity of RLS(△I) has linear relationship to the concentration of deoxyribonucleic acid.Thus a RLS method for determination of deoxyribonucleic acid is established.The linear range for determination of DNA is 0.053~1.20 μg·mL-1,the detection limit is 0.0159 μg·mL-1,and the recovery rate is 98.6%~102.3%.This method is simple,rapid and has been applied to determination of deoxyribonucleic acid in synthetic samples with satisfactory results.

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Key concepts: Chemistry, DNA, Orange (colour), Detection limit, Linear range, Linear relationship, Resonance (particle physics), Wavelength