2005•PubMedRequires access
[Determination of deoxyribonucleic acid with acridine orange by resonance light scattering technique].
Xinjun Wan, Jinshui Liu, Lun Wang
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Abstract
The resonance light scattering (RLS) spectra of acridine orange with deoxyribonucleic acid have been studied. The RLS of acridine orange is greatly enhanced by deoxyribonucleic acid in pH 11. There is a resonance light scattering peak at 324 nm. Under optimal conditions, the linear ranges of the calibration curves are 7.5 x 10(-8) -9.8 x 10(-6) g x mL(-1) for Calf thymus DNA (ct DNA), 3.1 x 10(-8) -7.3 x 10(-6) g x mL(-1) for Fish sperm DNA (fs DNA) with detection limits 20.8 ng x mL(-1) for ct DNA and 20.5 ng x mL(-1) for fs DNA. This method is simple, selectivity and sensitivity has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.