2003•PubMedRequires access

[Determination of deoxyribonucleic acid with alkali blue 6B by resonance light-scattering method].

Yunfei Long, Xiao-ming Chen, Qunli Wu, Weijun Yang

Abstract

Characteristics of the interaction between alkali blue 6B with deoxyribonucleic acid (DNA) was studied by resonance light-scattering (RLS) method in the pH 2.90 medium. The effects of interference substances and the optimization of procedure were investigated, and a new method based on RLS was established. The method can be used to determine the DNA at nanogram level. The linear range of the DNA is 10-100.4 micrograms.L-1 with a correlation coefficient of 0.9913. The detection limit is 4.2 micrograms-L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory results.

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Key concepts: Detection limit, Alkali metal, Resonance (particle physics), DNA, Scattering, Chemistry, Interference (communication), Correlation coefficient