Determination of N-di-Desmethylsibutramine with HPLC/ESI-MS in Slimming Health Food Products

Objective A high-performance liquid chromatography electrospray/mass spectrometry(HPLC/ESI-MS)method was developed to detect N-di-desmethylsibutramine in slimming health food products.Method The separation was completed on a RP-C_ l8 column(4.6 mm×250 mm,5 μm)with 0.25% acetic acid aqueous solution containing 20 mmmol/L ammonium acetate as mobile phase.Elution run at a flow rate of 1 ml/min and UV detection was performed at 223 nm.Results Good linearity was shown between the peak area and the concentration of N-di-desmethylsibutramine standards,in a range of 0.01-1.20 mg/ml.Conclusion The correlation coefficient of the calibration curves was better than 0.9997(n=6).The limit of detection(LOD)was 0.95 μg/ml(S/N=3).The method was sensitive,precise and highly reproducible.