Multiplex PCR for Detection of Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus 2(PCV-2)

Based on conserved sequences in ORF6 and ORF7 fragments of PRRSV American strain(ATCC VR-2332) and that in ORF2 of PCV-2(DQ195679)strain,two pairs of specific primers were respectively designed,through optimizing of RTPCR and PCR conditions,multiplex PCR for detection of PRRSV and PCV-2 was established.Fifteen organ samples from different farms of Henan province were respectively detected by the duplex PCR,out of which eight were PRRSV positive,five were PCV-2 positive,three were both PRRSV and PCV-2 positive,the control samples were negative,suggesting that the multiplex PCR of PRRSV and PCV-2 was a rapid, specific and sensitive diagnostic method.