Establishment and Application of Duplex PCR for PRRSV and PCV-2 Detection

Two pairs of specific primers were designed according to the sequences of PRRSV Nsp2 gene and PCV-2 ORF1 gene availiable on GenBank respectively.The amplified fragment of PRRSV was 421bp,and 714 bp for PCV-2.Two PCR/RT-PCR methods for detecting PRRSV and PCV-2 were developed using the two pairs of primers.Based on the two established PCR methods above,the duplex PCR method for detecting both PRRSV and PCV-2 was developed through combination and optimization of two methods.143 clinical samples were detected by both this duplex PCR method and two seperate PCR/RT-PCR methods.The results showed 92.6% consistency between the duplex PCR method and two seperate PCR/RT-PCR methods,which suggested that the established duplex PCR method can be used in clinical detection for PRRSV and PCV-2.