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2011•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination of Total Saponins in the Seeds of Impatiens Balsamina L.

Ju Cheng

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Abstract

The content of total saponins in the seeds of Impatiens balsamina L.was determined by spectrophotometry at 541nm with oleanolic acid as reference substance and colorating with vanillin-glacial acetic acid-perchloric acid.The calibration curve was linear in the range of 7.327—25.64μg/mL(r=0.9996).The average recovery of oleanolic acid was 98.7%(RSD=0.51%,n=6).The method is simple,accurate,sensitive.It can be applied to the quality control of the seeds of Impatiens balsamina L..

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The content of total saponins in the seeds of Impatiens balsamina L.was determined by spectrophotometry at 541nm with oleanolic acid as reference substance and colorating with vanillin-glacial acetic acid-perchloric acid.The calibration curve was linear in the range of 7.327—25.64μg/mL(r=0.9996).The average recovery of oleanolic acid was 98.7%(RSD=0.51%,n=6).The method is simple,accurate,sensitive.It can be applied to the quality control of the seeds of Impatiens balsamina L..

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Available abstract

The content of total saponins in the seeds of Impatiens balsamina L.was determined by spectrophotometry at 541nm with oleanolic acid as reference substance and colorating with vanillin-glacial acetic acid-perchloric acid.The calibration curve was linear in the range of 7.327—25.64μg/mL(r=0.9996).The average recovery of oleanolic acid was 98.7%(RSD=0.51%,n=6).The method is simple,accurate,sensitive.It can be applied to the quality control of the seeds of Impatiens balsamina L..

Key concepts: Impatiens, Oleanolic acid, Acetic acid, Chemistry, Calibration curve, Perchloric acid, Chromatography, Vanillin

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