Content Determination of Flavonoids in Impatiens balsamina L. by Spectrophotometry

[Objective] The research aimed to study the determination method of total flavonoids content in Impatiens balsamina L.by visible light and ultraviolet spectrophotometry.[Method] With rutin and kaempferol as reference substances respectively,by using visible light spectrophotometry and AlCl3 as chromogenic reagent,the content of total flavonoids was determined at the wavelength of 412 nm.By using ultraviolet spectrophotometry,the content of total flavonoids was determined at the wavelength of 270 nm.[Result] With rutin and kaempferol as reference substances respectively,the average recoveries by visible light method were 98.52% and 99.72%,with RSD of 2.38% and 3.14% resp.The average recoveries by visible light method were 97.53% and 98.75%,with RSD of 2.71% and 2.86% resp.[Coclution] By using visible light spectrophotometry,with kaempferol as reference substance is a kind of more ideal determination method of total flavonoids in Impatiens balsamina L..