2011•Journal of China West Normal UniversityRequires access

Optimizing on IFN-γ-induced ICAM-1 Expression in HaCaT Cells

Chunlian Wu

Abstract

Intercellular adhesion molecule-1(ICAM-1) was necessary for leukocyte and kerationocyte interaction.Upregulation of ICAM-1 expression in keratinocytes had correlation with many skin inflammation.The purpose of this study was to optimize conditions of IFN-γ-induced ICAM-1 expression on HaCaT cells in vitro.HaCaT cells were stimulated by IFN-γ at different concentration and time respectively.IFN-γ-induced ICAM-1 expression was detected in HaCaT cells by flow cytometry.Finally treatment of HaCaT cells with 1000U/mL IFN-γfor 24h markedly induced ICAM-1 expression.The results indicated that IFN-γ could up-regulate.The expression of ICAM-1 laid the foundation for skin inflammation therapeutics by using medicine latterly.

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Key concepts: HaCaT, ICAM-1, Flow cytometry, Inflammation, In vitro, Intercellular Adhesion Molecule-1, Downregulation and upregulation, Chemistry