Effects of FoxM1 on proliferation and cell cycle of HaCaT cells

Objective:To determine the effects of FoxMl on proliferation and cell cycle of HaCaT cells.Methods;Expression vectors of pEGFP-N2- FoxMl were constructed and HeLa cells were transfected with lipofectamine.HaCaT cells were re-cultured with different concentration of supernatant from cultured HeLa cells.The proliferation of HaCaT cells was detected by MTT assay.The cycle of HaCaT cells was detected by by flow cytometry.Results;FoxMl can promote proliferation of HaCaT cells and the higher the concentration,the more obvious the proliferation effect was.The proportion of cells in phase Gl significantly reduced,and the proliferation of cells in phase S and G2 significantly increased after re-culture with FoxMl.Conclusion;FoxM1 induces the proliferation of HaCaT cells in a dose dependent manner.