Effects of total glucosides of paeony on proliferation of human HaCaT keratinocytes and expression of ICAM-1

Objective To evaluate the effects of total glucosides of paeony(TGP) on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations(0.5 to 312.5 μg/ml). MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay(ELISA) and fluorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations(0.5 to 25.0 μg/ml), but inhibited by TGP of high concentration(62.5 to 125.0 μg/ml). TGP of low concentrations(0.5 to 25.0 μg/mL) significantly reduced the expression of ICAM-1. Conclusion TGP significantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inflammatory mechanism of TGP.